Molecular Mondays with Raina

Visual comparison of qPCR vs ELISA in allergen testing, showing qPCR for allergen DNA verification and ELISA for allergen protein detection.

qPCR vs ELISA in Allergen Testing: When to Use Which, and Why

Allergen testing requires the right tool for the right question. This post explains qPCR vs ELISA in allergen testing, when to use each method, and how both support effective allergen control and confident decision-making.

3 days ago3 min read

Environmental swab sampling for qPCR-based allergen verification during cleaning validation in a food processing facility.

qPCR for Allergen Verification in Food

Allergen management goes beyond labels and supplier declarations. This post explains how qPCR for allergen verification supports cleaning validation, cross-contact assessment, and evidence-based allergen control in food manufacturing.

3 days ago2 min read

qPCR amplification curves showing detected signals, illustrating how qPCR positives require careful interpretation before concluding contamination.

qPCR Positives: When a “Detected” Result Does NOT Mean Contamination

A “Detected” result in qPCR often triggers immediate concern, but detection does not always equal contamination. This post explains what qPCR positives truly mean across microbial, GMO, and allergen testing — and how correct interpretation prevents unnecessary panic and wrong quality decisions.

Jan 53 min read

qPCR amplification curves illustrating shifts in Ct values that indicate early spoilage detection before visible colony growth on plates.

🧬Early Spoilage Detection Through Ct Shifts: How qPCR Detects Spoilage Before Plates Do

Learn how Early Spoilage Detection Through Ct Shifts using qPCR lets food labs identify spoilage before plate growth appears — enabling faster action and better quality control.

Dec 22, 20253 min read

qPCR amplification curves showing Ct value variation and explaining why two laboratories never get the same Ct value

🧬Why Two Labs Never Get the Same Ct Value (Understanding Ct Value Variation)

Learn why two labs never report the same Ct value and how Ct value variation affects qPCR interpretation. A practical guide for food industry QA teams.

Dec 15, 20252 min read

A vertically stacked collage showing qPCR consumables in the upper image and microbial colonies on agar plates in the lower image, visually contrasting rapid molecular detection with traditional culture methods.

🧬 qPCR vs Plating: Why Molecular Methods Are Changing Pathogen Testing - When it shouldn't

Why qPCR Is Changing Pathogen Testing — and When It Shouldn’t Replace Plating

Dec 8, 20253 min read

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