Ct value | 2fquality

qPCR amplification curves showing detected signals, illustrating how qPCR positives require careful interpretation before concluding contamination.

qPCR Positives: When a “Detected” Result Does NOT Mean Contamination

A “Detected” result in qPCR often triggers immediate concern, but detection does not always equal contamination. This post explains what qPCR positives truly mean across microbial, GMO, and allergen testing — and how correct interpretation prevents unnecessary panic and wrong quality decisions.

Jan 53 min read

qPCR amplification curves illustrating shifts in Ct values that indicate early spoilage detection before visible colony growth on plates.

🧬Early Spoilage Detection Through Ct Shifts: How qPCR Detects Spoilage Before Plates Do

Learn how Early Spoilage Detection Through Ct Shifts using qPCR lets food labs identify spoilage before plate growth appears — enabling faster action and better quality control.

Dec 22, 20253 min read

qPCR amplification curves showing Ct value variation and explaining why two laboratories never get the same Ct value

🧬Why Two Labs Never Get the Same Ct Value (Understanding Ct Value Variation)

Learn why two labs never report the same Ct value and how Ct value variation affects qPCR interpretation. A practical guide for food industry QA teams.

Dec 15, 20252 min read

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